The Bolitoglossa macrinii species group (restricted geographically to southern Oaxaca and southwestern Guerrero, México) is a monophyletic assemblage of five species, B. macrinii, Bolitoglossa riletti, Bolitoglossa hermosa, Bolitoglossa oaxacensis sp. nov., and Bolitoglossa zapoteca sp. nov. DNA sequences totaling 1164 base pairs for the mitochondrial genes 16S ribosomal RNA and cytochrome b were analyzed to generate a phylogenetic hypothesis for the relationships within the group. Our hypothesis is in agreement with previous morphological and allozyme analyses. Divergence within the group is great (to 18.6% for cytochrome b, to 7.0% for 16S rRNA) and two clades are well supported: one including B. riletti, B. hermosa, and B. zapoteca and the other including B. macrinii and B. oaxacensis. These clades likely diverged beginning in mid-Miocene times. We describe two new species, B. oaxacensis, for inland Oaxacan populations previously assigned to B. macrinii, and B. zapoteca for the easternmost populations of the clade. The new species are diagnosed by less interdigital webbing, distinctive coloration (each with subdued or no white spotting but differing from each other in pattern and hue) and by extensive differentiation in allozymes (B. oaxacensis) and mitochondrial DNA sequences (both species). The new taxa are the third and fourth species of Bolitoglossa endemic to the State of Oaxaca.